The experiment involved culturing R28 cells in a controlled environment outside their natural body, exposing them to different light sources with different spectral characteristics and measuring the number of live cells over time.
Three commonly used commercial luminaires were selected: a cold white standard LED, a neutral white full-spectrum LED with purple primary energy, and a neutral white full-spectrum LED with blue primary energy, and their effect on R28 cell cultivation was compared to a Spectrasol procognitive full-spectrum LED light source with contained red photobiomodulation energy.
Spectrasol procognitive LED luminaire with red reparation light component on the human eye as an effective tool for the protection and regeneration of the retinal cells of the eye
The experiment highlights the importance of the spectral power distribution of light indoors, particularly in relation to its biological effects on retinal cells. The commercial lights under comparison caused cell apoptosis after approximately
200 minutes of illumination, while only the Spectrasol light source showed an almost twofold increase in the number of living cells after 60 minutes of illumination after the initial reduction. The results show that the Spectrasol light source with a red LED chip (670 nm) neutralises the negative effects of blue light on retinal cells and improves their vitality.
Exposure to short-wave light sources, which are common in commercial LED lighting, can have potentially harmful effects on the visual system
The experiment shows that irradiation of R28 retinal cells with short-wave radiation from other tested LED lamps caused their apoptosis without subsequent regeneration and therefore long-term use of these lamps may have irreversible negative effects on retinal cells of the eye.